Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples
نویسنده
چکیده
Equine aural plaques were recently associated with four equine papillomaviruses (EcPVs) (Lange et al., 2013; Taniwaki et al., 2013). These viruses have been detected in fresh samples by PCR (Lange et al. 2013; Taniwaki et al., 2013), but EcPVs have not been identified in formalinfixed and paraffin-embedded (FFPE) aural plaque samples. Archives of FFPE aural plaques and other papilloma specimens stored in diverse veterinary diagnostic centers may represent a valuable source of material for the etiologic diagnosis of papillomaviruses involved in the disease (Newkirk et al., 2014); therefore, the aim of this study was to optimize a reliable method for the detection of the EcPVs in FFPE aural plaque samples by PCR.
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